Device

Part:BBa_K173007

Designed by: Lorenzo Pasotti, Letizia Diamante, Paolo Magni   Group: iGEM09_UNIPV-Pavia   (2009-10-15)

aTc inducible system (aTc->PoPS) with J23100 promoter

This BioBrick is an [http://openwetware.org/wiki/ATc aTc] input -> PoPS output device.

BBa_J23100 promoter drives the constitutive production of tetR repressor (BBa_C0040), which inhibits tetR promoter (BBa_R0040) activity. When aTc is added to the medium, it binds tetR and inhibits it. So, the PoPS output is a function of the aTc concentration.

Pv A6 BBa K173007.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None